验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, SH-5Y5Y cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83833-5-RR targets PTPRS in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg0576 Product name: Recombinant human rPTPσ protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 825-1000 aa of NM_002850 Sequence: VVVTKGAVLGRPTLSVQQTPEGSLLARWEPPAGTAEDQVLGYRLQFGREDSTPLATLEFPPSEDRYTASGVHKGATYVFRLAARSRGGLGEEAAEVLSIPEDTPRGHPQILEAAGNASAGTVLLRWLPPVPAERNGAIVKYTVAVREAGALGPARETELPAAAEPGAENALTLQGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, S |
| 别名 | 241005B6, EC:3.1.3.48, PTPSIGMA, R PTP S, R PTP sigma |
| 计算分子量 | 217 kDa |
| 观测分子量 | 140 kDa, 217 kDa |
| GenBank蛋白编号 | NM_002850 |
| 基因名称 | PTPRS |
| Gene ID (NCBI) | 5802 |
| RRID | AB_3671416 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q13332 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tyrosine phosphatase receptor type S (PTPRS, also known as R-PTP-S, PTPSIGMA, and R-PTP-sigma) is an integral membrane protein. It can promote oligomerization by binding to large heparan sulfate proteoglycan structures, and interact with NTRK1/3, PPFIA1/2/3 (PMID: 25385546; 8524829; 9624153). The non-glycosylated molecular weight of PTPRS is 214 kDa, and the extracellular domain can be cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Variations and mutations of PTPRS are associated with Alzheimer's disease and cholangiocarcinoma (PMID: 38766183; 35991009).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for PTPRS antibody 83833-5-RR | Download protocol |
| WB protocol for PTPRS antibody 83833-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
