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验证数据展示

  • WB analysis using 30763-1-AP

    WB analysis using 30763-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30763-1-AP (PTPRM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30763-1-AP

    WB analysis using 30763-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30763-1-AP (PTPRM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 30763-1-AP

    IHC staining of mouse kidney using 30763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30763-1-AP (PTPRM/RPTPmu antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PTPRM/RPTPmu Polyclonal antibody

PTPRM/RPTPmu Polyclonal Antibody for WB, ELISA
Cat No. 30763-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

PTPRM, EC:3.1.3.48, hR PTPu, Protein-tyrosine phosphatase mu, PTPRL1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells, U-87 MG cells, rat eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30763-1-AP targets PTPRM/RPTPmu in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag33475

Product name: Recombinant human PTPRM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 765-880 aa of NM_002845

Sequence: KKRKLAKKRKETMSSTRQEMTVMVNSMDKSYAEQGTNCDEAFSFMDTHNLNGRSVSSPSSFTMKTNTLSTSVPNSYYPDETHTMASDTSSLVQSHTYKKREPADVPYQTGQLHPAI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, M
别名 PTPRM, EC:3.1.3.48, hR PTPu, Protein-tyrosine phosphatase mu, PTPRL1
计算分子量164KD
观测分子量80 kDa, 100 kDa, 200 kDa
GenBank蛋白编号NM_002845
基因名称 PTPRM
Gene ID (NCBI) 5797
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28827
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRM, also known as protein tyrosine phosphatase receptor type M or RPTPmu, is a protein with a molecular weight of approximately 155 kDa. It is a receptor-type tyrosine phosphatase involved in cell-cell adhesion, migration, and signaling. It exists in two forms, either a full-length 200 kDa form or as two noncovalently associated fragments of 100 kDa each.Additional unidentified serine proteases cleave PTPμ in GBM cells to yield smaller extracellular fragments with MWs of 78 and 55 kDa (PMID: 25223585).

实验方案

Product Specific Protocols
IHC protocol for PTPRM/RPTPmu antibody 30763-1-APDownload protocol
WB protocol for PTPRM/RPTPmu antibody 30763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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