×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of A549 using 12109-1-AP

    WB analysis of A549 using 12109-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12109-1-AP

    IP experiment of HEK-293 using 12109-1-AP

    IP result of anti-PTPN9 (IP:12109-1-AP, 4ug; Detection:12109-1-AP 1:300) with HEK-293 cells lysate 1800ug.

  • IHC staining of human liver cancer using 12109-1-AP

    IHC staining of human liver cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN9 antibody included
  • IHC staining of human liver cancer using 12109-1-AP

    IHC staining of human liver cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN9 antibody included
  • WB analysis using 12109-1-AP

    WB analysis using 12109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12109-1-AP

    IHC staining of human colon cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12109-1-AP (PTPN9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN9 antibody included
  • IHC staining of human placenta using 12109-1-AP

    IHC staining of human placenta using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12109-1-AP (PTPN9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN9 antibody included

PTPN9 Polyclonal antibody

PTPN9 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12109-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

MEG2, EC:3.1.3.48, Protein-tyrosine phosphatase MEG2, PTPase MEG2, PTPaseMEG2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, Transfected HEK-293 cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, rat kidney tissue, mouse kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® PTPN9 Ready-To-Use IHC Kit. PTPN9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12109-1-AP targets PTPN9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag2754

Product name: Recombinant human PTPN9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 248-593 aa of BC010863

Sequence: FQFLPQVNGHPDPFDEIILFSLPPALDWDSVHVPGPHAMTIQELVDYVNARQKQGIYEEYEDIRRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGHTQTDYINASFMDGYKQKNAYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKCGQYWPLEKDSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYGVPSSAASLIDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICLAQLEELGTLNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVESQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 9
别名 MEG2, EC:3.1.3.48, Protein-tyrosine phosphatase MEG2, PTPase MEG2, PTPaseMEG2
计算分子量 593 aa, 68 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC010863
基因名称 PTPN9
Gene ID (NCBI) 5780
RRIDAB_2877826
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43378
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).

实验方案

Product Specific Protocols
IHC protocol for PTPN9 antibody 12109-1-APDownload protocol
IP protocol for PTPN9 antibody 12109-1-APDownload protocol
WB protocol for PTPN9 antibody 12109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

MicroRNA-126 Regulates Angiogenesis and Neurogenesis in a Mouse Model of Focal Cerebral Ischemia.

Authors - Meijie Qu
View Article
humanWB

FASEB J

PTPN9 promotes melanoma progression by regulating the ferroptosis pathway

Authors - Hongmei Wang
  • KD Validated
View Article
||
New chat

Able™

正在加载，请稍候...