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验证数据展示

  • WB analysis using 15286-1-AP (same clone as 15286-1-PBS)

    WB analysis using 15286-1-AP (same clone as 15286-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

PTPN7 Polyclonal antibody, PBS Only

PTPN7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15286-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15286-1-PBS targets PTPN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7453

Product name: Recombinant human PTPN7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-399 aa of BC001746

Sequence: MGASFWPIRQAREQQRRALSFRQTSWLSEPPLGPAPHLSMVQAHGGRSRAQPLTLSLGAAMTQPPPEKTPAKKHVRLQERRGSNVALMLDVRSLGAVEPICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEFLKIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGPMPNTVSDFWEMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQYQEERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGCFIATRIGCQQLKARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 7
别名
计算分子量 41 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC001746
基因名称 PTPN7
Gene ID (NCBI) 5778
RRIDAB_2173831
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35236
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

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