• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PTPN7 Polyclonal antibody

PTPN7 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15286-1-AP

Print datasheet

Synonyms

BPTP 4, HEPTP, LC PTP, LPTP, PTPN7, PTPNI

验证数据展示

  • WB analysis using 15286-1-AP

    WB analysis using 15286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 15286-1-AP

    IHC staining of human tonsillitis using 15286-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 15286-1-AP

    IHC staining of human tonsillitis using 15286-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 15286-1-AP

    IHC staining of human tonsillitis using 15286-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15286-1-AP targets PTPN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Immunogen PTPN7 fusion protein Ag7453 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 7
Synonyms BPTP 4, HEPTP, LC PTP, LPTP, PTPN7, PTPNI
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC001746
Gene Symbol PTPN7
Gene ID (NCBI) 5778
RRIDAB_2173831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35236
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

实验方案

Product Specific Protocols
WB protocol for PTPN7 antibody 15286-1-APDownload protocol
IHC protocol for PTPN7 antibody 15286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Front Cell Dev Biol

piR-121380 Is Involved in Cryo-Capacitation and Regulates Post-Thawed Boar Sperm Quality Through Phosphorylation of ERK2 via Targeting PTPN7.

Authors - Yihan Wang
View Article
humanIHC

Front Genet

Comprehensive analysis of PTPN gene family revealing PTPN7 as a novel biomarker for immuno-hot tumors in breast cancer

Authors - Fengxu Wang
View Article