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CoraLite® Plus 488-conjugated PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-24546

Print datasheet

Synonyms

HCP, HCPH, HPTP1C, PTP 1C, PTP1C, PTPN6, SH PTP1, SHP 1, SHP 1L, SHP1

验证数据展示

  • IF Staining of MCF-7 using CL488-24546

    IF Staining of MCF-7 using CL488-24546

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PTPN6 antibody (CL488-24546) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-24546

    FC experiment of MCF-7 using CL488-24546

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTPN6 (CL488-24546) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 24546-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24546 targets PTPN6 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra)
Tested Reactivity human
Immunogen PTPN6 fusion protein Ag21415 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 6
Synonyms HCP, HCPH, HPTP1C, PTP 1C, PTP1C, PTPN6, SH PTP1, SHP 1, SHP 1L, SHP1
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC002523
Gene Symbol PTPN6
Gene ID (NCBI) 5777
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29350
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PTPN6 antibody CL488-24546Download protocol
FC protocol for CL Plus 488 PTPN6 antibody CL488-24546Download protocol
Standard Protocols
Click here to view our Standard Protocols