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验证数据展示

  • IF Staining of mouse heart using CL647-10472

    IF Staining of mouse heart using CL647-10472

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®647 PTPN23 antibody (CL647-10472) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using CL647-10472

    IF Staining of HepG2 using CL647-10472

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®647 PTPN23 antibody (CL647-10472) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®647-conjugated PTPN23 Polyclonal antibody

PTPN23 Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL647-10472

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P

别名

EC:3.1.3.48, HD PTP, HDPTP, HD-PTP, His domain-containing protein tyrosine phosphatase

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®647
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10472-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10472 targets PTPN23 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag0765

Product name: Recombinant human PTPN23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 420-619 aa of BC004881

Sequence: QEKLHLRFCYEAVVRHVEQVLQRHGVPPPCKPLASASISQKNHLPQDSQDLVLGGDVPISSIQATIAKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPLPEAPQPKEEPPVPEAPSSGPPSSSLELLASLTPEAFSLDSSLRGKQRMSKHNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 23
别名 EC:3.1.3.48, HD PTP, HDPTP, HD-PTP, His domain-containing protein tyrosine phosphatase
计算分子量 179 kDa
观测分子量 179 kDa
GenBank蛋白编号BC004881
基因名称 PTPN23
Gene ID (NCBI) 25930
RRIDAB_3084801
偶联类型 CoraLite®647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3S7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).

实验方案

Product Specific Protocols
IF protocol for CL647 PTPN23 antibody CL647-10472Download protocol
Standard Protocols
Click here to view our Standard Protocols
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