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验证数据展示

  • WB analysis using 83024-1-RR

    WB analysis using 83024-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83024-1-RR (PTPN18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83024-1-RR

    FC experiment of HEK-293 using 83024-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PTPN18 Recombinant antibody (83024-1-RR, Clone:230154G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83024-1-RR

    Affinity and Kinetic Characterization of 83024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83024-1-RR against Human PTPN18 were performed. The affinity constant is 7.48 nM.

PTPN18 Recombinant monoclonal antibody

PTPN18 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83024-1-RR

产品说明书

CloneNo. 230154G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, T-47D cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83024-1-RR targets PTPN18 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34048

Product name: Recombinant human PTPN18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-351 aa of BC052800

Sequence: VQKRGAPAGAGSGTQTGTGTGARSAEEAPLYSKVTPRAQRPGAHAEDARGTLPGRVPADQSPAGSGAYEDVAGGAQTGGLGFNLRIGRPKGPRDPPAEWTRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 18 (brain-derived)
别名 Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1
计算分子量 460 aa, 50 kDa
观测分子量48 kDa
GenBank蛋白编号BC052800
基因名称 PTPN18
Gene ID (NCBI) 26469
RRIDAB_3670764
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99952
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN18 (also called PTP-HSCF or BDP1), a member of the protein tyrosine phosphatase superfamily, has been reported to be a tumor suppressor in breast cancer by dephosphorylating HER2 and suppressing cell proliferation and invasion. In addition to the well-established role of PTPN18 in breast cancer, our previous study found that PTPN18 and CSK cooperate to inhibit the events triggered by SRC kinases. (PMID: 35982039)

实验方案

Product Specific Protocols
FC protocol for PTPN18 antibody 83024-1-RRDownload protocol
WB protocol for PTPN18 antibody 83024-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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