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验证数据展示

  • WB analysis using 85810-1-RR (same clone as 85810-1-PBS)

    WB analysis using 85810-1-RR (same clone as 85810-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85810-1-RR (PTGIS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85810-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 85810-1-RR (same clone as 85810-1-PBS)

    IF Staining of U2OS using 85810-1-RR (same clone as 85810-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTGIS antibody (85810-1-RR, Clone: 243128C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85810-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85810-1-RR

    Affinity and Kinetic Characterization of 85810-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85810-1-RR against Human PTGIS were performed. The affinity constant is below 1 pM.

PTGIS Recombinant monoclonal antibody, PBS Only

PTGIS Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85810-1-PBS

产品说明书

CloneNo. 243128C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

CYP8, CYP8A1, EC:4.2.1.152, EC:5.3.99.4, Hydroperoxy icosatetraenoate dehydratase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85810-1-PBS targets PTGIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25817

Product name: Recombinant human PTGIS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-285 aa of BC101809

Sequence: AIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prostaglandin I2 (prostacyclin) synthase
别名 CYP8, CYP8A1, EC:4.2.1.152, EC:5.3.99.4, Hydroperoxy icosatetraenoate dehydratase
计算分子量 500 aa, 57 kDa
观测分子量50-57 kDa
GenBank蛋白编号BC101809
基因名称 PTGIS
Gene ID (NCBI) 5740
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16647
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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