验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, HL-60 cells, mouse brain tissue |
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24895-1-AP targets PTGER4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
CatNo: Ag19217 Product name: Recombinant human PTGER4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 333-488 aa of BC101534 Sequence: RKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSESVLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prostaglandin E receptor 4 (subtype EP4) |
| 别名 | EP4R, EP4 |
| 计算分子量 | 488 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC101534 |
| 基因名称 | PTGER4 |
| Gene ID (NCBI) | 5734 |
| RRID | AB_2879782 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35408 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Abnormal mitochondrial iron metabolism damages alveolar type II epithelial cells involved in bleomycin-induced pulmonary fibrosis | ||
J Clin Invest Antibiotic pretreatment alleviates liver transplant damage in mice and humans. | ||
Adv Sci (Weinh) Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling. | ||
Cell Commun Signal PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cells | ||
J Orthop Translat Sensory nerve EP4 facilitates heterotopic ossification by regulating angiogenesis-coupled bone formation | ||
Mol Med Hyperoxia-activated Nrf2 regulates ferroptosis in intestinal epithelial cells and intervenes in inflammatory reaction through COX-2/PGE2/EP2 pathway |
