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验证数据展示

  • IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTEN antibody (80718-7-RR, Clone: 240134F7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PTEN Recombinant antibody (80718-7-RR, Clone:240134F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80718-7-PBS

    Affinity and Kinetic Characterization of 80718-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 80718-7-RR against Human PTEN were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

PTEN Recombinant monoclonal antibody, PBS Only

PTEN Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 80718-7-PBS

产品说明书

CloneNo. 240134F7

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC, FC (Intra), ELISA

别名

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.

产品信息

80718-7-PBS targets PTEN in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag17274

Product name: Recombinant human PTEN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 204-403 aa of BC005821

Sequence: PMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatase and tensin homolog
别名 10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1
计算分子量 47 kDa
GenBank蛋白编号BC005821
基因名称 PTEN
Gene ID (NCBI) 5728
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP60484
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C