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验证数据展示

  • WB analysis using 33489-1-AP

    WB analysis using 33489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33489-1-AP (PTCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 33489-1-AP

    IHC staining of rat brain using 33489-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 33489-1-AP (PTCHD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 33489-1-AP

    IHC staining of rat brain using 33489-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 33489-1-AP (PTCHD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PTCHD4 Polyclonal antibody

PTCHD4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33489-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C6orf138, p53-regulated patched protein, Patched domain-containing protein 4, PTCH53

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33489-1-AP targets PTCHD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37091

Product name: Recombinant human C6orf138 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 150-323 aa of BC137359

Sequence: SDGANIINLLASDSPSVSYAMVQQKYFSNYSPVIGFYVYEPLEYWNSSVQDDLRRLCSGFTAVSWVEQYYQFLKVSNVSANNKSDFISVLQSSFLKKPEFQHFRNDIIFSKAGDESNIIASRLYLVARTSRDKQKEITEVLEKLRPLSLSKSIRFIVFNPSFVFMDHYSLSVTV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 138
别名 C6orf138, p53-regulated patched protein, Patched domain-containing protein 4, PTCH53
观测分子量90 kDa
GenBank蛋白编号BC137359
基因名称 C6orf138
Gene ID (NCBI) 442213
RRIDAB_3742990
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZW05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTCHD4 (Patched Domain-Containing Protein 4; formerly C6orf138) is a 96 kDa multi-pass transmembrane protein encoded at 6p12.3 that belongs to the Patched family of cholesterol/lipid transporters .

实验方案

Product Specific Protocols
IHC protocol for PTCHD4 antibody 33489-1-APDownload protocol
WB protocol for PTCHD4 antibody 33489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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