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persephin-Specific Polyclonal antibody

persephin-Specific Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19713-1-AP

Print datasheet

Synonyms

persephin, PSP, PSPN

验证数据展示

  • WB analysis of SH-SY5Y using 19713-1-AP

    WB analysis of SH-SY5Y using 19713-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19713-1-AP

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 19713-1-AP

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of SH-SY5Y using 19713-1-AP

    IF Staining of SH-SY5Y using 19713-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19713-1-AP targets persephin-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name persephin
Synonyms persephin, PSP, PSPN
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberNM_004158
Gene Symbol PSPN
Gene ID (NCBI) 5623
RRIDAB_10640431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60542
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.

实验方案

Product Specific Protocols
WB protocol for persephin-Specific antibody 19713-1-APDownload protocol
IHC protocol for persephin-Specific antibody 19713-1-APDownload protocol
IF protocol for persephin-Specific antibody 19713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biol Cell

Vps15p regulates the distribution of cup-shaped organelles containing the major eisosome protein Pil1p to the extracellular fraction required for endocytosis of extracellular vesicles carrying metabolic enzymes.

Authors - Kathryn Stein
View Article