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验证数据展示

  • WB analysis using 19713-1-AP

    WB analysis using 19713-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19713-1-AP

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary cancer using 19713-1-AP

    IHC staining of human ovary cancer using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 19713-1-AP

    IHC staining of human colon cancer using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 19713-1-AP

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 19713-1-AP

    IF Staining of SH-SY5Y using 19713-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using persephin-Specific antibody (19713-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

persephin-Specific Polyclonal antibody

persephin-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19713-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

persephin, PSPN, PSP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19713-1-AP targets persephin-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 persephin
别名 persephin, PSPN, PSP
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号NM_004158
基因名称 Persephin/PSPN
Gene ID (NCBI) 5623
RRIDAB_10640431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60542
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.

实验方案

Product Specific Protocols
WB protocol for persephin-Specific antibody 19713-1-APDownload protocol
IHC protocol for persephin-Specific antibody 19713-1-APDownload protocol
IF protocol for persephin-Specific antibody 19713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biol Cell

Vps15p regulates the distribution of cup-shaped organelles containing the major eisosome protein Pil1p to the extracellular fraction required for endocytosis of extracellular vesicles carrying metabolic enzymes.

Authors - Kathryn Stein
View Article