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验证数据展示

  • WB analysis using 19713-1-AP

    WB analysis using 19713-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19713-1-AP

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary cancer using 19713-1-AP

    IHC staining of human ovary cancer using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 19713-1-AP

    IHC staining of human colon cancer using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 19713-1-AP

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse brain using 19713-1-AP

    IHC staining of mouse brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 19713-1-AP

    IHC staining of mouse brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 19713-1-AP

    IF Staining of SH-SY5Y using 19713-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using persephin-Specific antibody (19713-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of SH-SY5Y using 19713-1-AP

    FC experiment of SH-SY5Y using 19713-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

  • FC experiment of HepG2 using 19713-1-AP

    FC experiment of HepG2 using 19713-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

persephin-Specific Polyclonal antibody

persephin-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 19713-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

persephin, PSPN, PSP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human ovary cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 ul suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19713-1-AP targets persephin-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 persephin
别名 persephin, PSPN, PSP
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号NM_004158
基因名称 Persephin/PSPN
Gene ID (NCBI) 5623
RRIDAB_10640431
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60542
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.

实验方案

Product Specific Protocols
WB protocol for persephin-Specific antibody 19713-1-APDownload protocol
IHC protocol for persephin-Specific antibody 19713-1-APDownload protocol
IF protocol for persephin-Specific antibody 19713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Signal Transduct Target Ther

Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma

Authors - Minghao Xu
View Article
WB

Biol Cell

Vps15p regulates the distribution of cup-shaped organelles containing the major eisosome protein Pil1p to the extracellular fraction required for endocytosis of extracellular vesicles carrying metabolic enzymes.

Authors - Kathryn Stein
View Article
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