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验证数据展示

  • IF Staining of HeLa using CL488-67466

    IF Staining of HeLa using CL488-67466

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PSMG3 antibody (CL488-67466, Clone: 2H9C3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67466

    FC experiment of HeLa using CL488-67466

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PSMG3 Monoclonal antibody (CL488-67466, Clone:2H9C3)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated PSMG3 Monoclonal antibody

PSMG3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67466

产品说明书

CloneNo. 2H9C3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

C7orf48, hPAC3, PAC 3, PAC3, PAC-3

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67466-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67466 targets PSMG3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag8384

Product name: Recombinant human PSMG3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-122 aa of BC004308

Sequence: MEDTPLVISKQKTEVVCGVPTQVVCTAFSSHILVVVTQFGKMGTLVSLEPSSVASDVSKPVLTTKVLLGQDEPLIHVFAKNLVAFVSQEAGNRAVLLAVAVKDKSMEGLKALREVIRVCQVW

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) assembly chaperone 3
别名 C7orf48, hPAC3, PAC 3, PAC3, PAC-3
计算分子量 13 kDa
GenBank蛋白编号BC004308
基因名称 PSMG3
Gene ID (NCBI) 84262
RRIDAB_3672950
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BT73
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMG3, also named as C7orf48 and PAC3, is a chaperone protein which promotes assembly of the 20S proteasome. It may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PSMG3 antibody CL488-67466Download protocol
IF protocol for CL Plus 488 PSMG3 antibody CL488-67466Download protocol
Standard Protocols
Click here to view our Standard Protocols
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