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验证数据展示

  • IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

    IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSME4 antibody included
  • IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

    IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSME4 antibody included
  • WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    IP experiment of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    IP result of anti-PSME4 (IP:18799-1-AP, 3ug; Detection:18799-1-AP 1:500) with HEK-293 cells lysate 1760ug. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

    IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PSME4 Polyclonal antibody, PBS Only

PSME4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18799-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSME4 Ready-To-Use IHC Kit. PSME4 primary antibody included.

产品信息

18799-1-PBS targets PSME4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) activator subunit 4
别名
计算分子量 211 kDa
观测分子量 140 kDa
GenBank蛋白编号BC113668
基因名称 PSME4
Gene ID (NCBI) 23198
RRIDAB_10598020
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14997
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.

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