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验证数据展示

  • IHC staining of human liver cancer using 18799-1-AP

    IHC staining of human liver cancer using 18799-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME4 antibody included
  • IHC staining of human liver cancer using 18799-1-AP

    IHC staining of human liver cancer using 18799-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME4 antibody included
  • WB analysis of HEK-293 using 18799-1-AP

    WB analysis of HEK-293 using 18799-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 18799-1-AP

    IP experiment of HEK-293 using 18799-1-AP

    IP result of anti-PSME4 (IP:18799-1-AP, 3ug; Detection:18799-1-AP 1:500) with HEK-293 cells lysate 1760ug.

  • IF Staining of HepG2 using 18799-1-AP

    IF Staining of HepG2 using 18799-1-AP

    Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Featured Product
  • KD/KO验证

PSME4 Polyclonal antibody

PSME4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18799-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

PSME 4, KIAA0077

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Planning an IHC experiment? We recommend our IHCeasy PSME4 Ready-To-Use IHC Kit. PSME4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18799-1-AP targets PSME4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) activator subunit 4
别名 PSME 4, KIAA0077
计算分子量 211 kDa
观测分子量 140 kDa
GenBank蛋白编号BC113668
基因名称 PSME4
Gene ID (NCBI) 23198
RRIDAB_10598020
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14997
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.

实验方案

Product Specific Protocols
WB protocol for PSME4 antibody 18799-1-APDownload protocol
IHC protocol for PSME4 antibody 18799-1-APDownload protocol
IF protocol for PSME4 antibody 18799-1-APDownload protocol
IP protocol for PSME4 antibody 18799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

EBioMedicine

CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy

Authors - Peixia Tang
View Article
humanIHC

EBioMedicine

Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.

Authors - Zhen Yu
  • KD Validated
View Article
humanWB

Int J Gen Med

PSME4 Activates mTOR Signaling and Promotes the Malignant Progression of Hepatocellular Carcinoma.

Authors - Sijia Ge
  • KD Validated
View Article