验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, human brain tissue, mouse pancreas tissue, human kidney tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12937-2-AP targets PSME2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PSME2 fusion protein Ag3986 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) activator subunit 2 (PA28 beta) |
| 别名 | REG-beta, REGbeta, REG beta, PA28beta, PA28B |
| 计算分子量 | 239 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC019885 |
| 基因名称 | PSME2 |
| Gene ID (NCBI) | 5721 |
| RRID | AB_2300265 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL46 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSME2 antibody 12937-2-AP | Download protocol |
| IHC protocol for PSME2 antibody 12937-2-AP | Download protocol |
| IF protocol for PSME2 antibody 12937-2-AP | Download protocol |
| IP protocol for PSME2 antibody 12937-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Biol Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization | ||
Front Genet PSME2 identifies immune-hot tumors in breast cancer and associates with well therapeutic response to immunotherapy | ||
Pharmacol Res PD-L1 upregulation promotes drug-induced pulmonary fibrosis by inhibiting vimentin degradation |
