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PSMD2 Monoclonal antibody

PSMD2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1G7G5

Cat No : 67770-1-Ig

Print datasheet

Synonyms

26S proteasome subunit p97, P97, Protein 55.11, PSMD2, Rpn1, S2, TRAP2

验证数据展示

  • WB analysis using 67770-1-Ig

    WB analysis using 67770-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67770-1-Ig (PSMD2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67770-1-Ig targets PSMD2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,ELISA
Tested Reactivity Human, mouse, rat
Immunogen PSMD2 fusion protein Ag6543 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Synonyms 26S proteasome subunit p97, P97, Protein 55.11, PSMD2, Rpn1, S2, TRAP2
Calculated Molecular Weight 100 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC002368
Gene Symbol PSMD2
Gene ID (NCBI) 5708
RRIDAB_2918536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13200
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor necrosis factor type 1 receptor-associated protein 2 (TRAP2), encoded by PSMD2 gene, is a non-ATPase regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. TRAP2 may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor.

实验方案

Product Specific Protocols
WB protocol for PSMD2 antibody 67770-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols