• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86596-3-RR (same clone as 86596-3-PBS)

    WB analysis using 86596-3-RR (same clone as 86596-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86596-3-RR (PSMD11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86596-3-RR (same clone as 86596-3-PBS)

    IF Staining of HeLa using 86596-3-RR (same clone as 86596-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD11 antibody (86596-3-RR, Clone: 251499B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 86596-3-RR (same clone as 86596-3-PBS)

    IHC staining of mouse testis using 86596-3-RR (same clone as 86596-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86596-3-RR (PSMD11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMD11 antibody included

PSMD11 Recombinant monoclonal antibody, PBS Only

PSMD11 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86596-3-PBS

产品说明书

CloneNo. 251499B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

26S proteasome non-ATPase regulatory subunit 11, p44.5, Rpn6, S9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PSMD11 Ready-To-Use IHC Kit. PSMD11 primary antibody included.

产品信息

86596-3-PBS targets PSMD11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6435

Product name: Recombinant human PSMD11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 76-422 aa of BC000437

Sequence: FLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
别名 26S proteasome non-ATPase regulatory subunit 11, p44.5, Rpn6, S9
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC000437
基因名称 PSMD11
Gene ID (NCBI) 5717
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00231
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.

||
New chat Able
正在加载，请稍候...