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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 86120-2-RR (same clone as 86120-2-PBS)

    WB analysis using 86120-2-RR (same clone as 86120-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86120-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 86120-2-RR (same clone as 86120-2-PBS)

    IHC staining of human colon cancer using 86120-2-RR (same clone as 86120-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86120-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMD10 antibody included

PSMD10/Gankyrin Recombinant antibody, PBS Only

PSMD10/Gankyrin Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86120-2-PBS

产品说明书

CloneNo. 250170D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

PSMD10, 26S proteasome non-ATPase regulatory subunit 10, Gankyrin, p28, p28(GANK)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMD10 Ready-To-Use IHC Kit. PSMD10 primary antibody included.

产品信息

86120-2-PBS targets PSMD10/Gankyrin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 PSMD10/Gankyrin fusion protein Ag2994 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
别名 PSMD10, 26S proteasome non-ATPase regulatory subunit 10, Gankyrin, p28, p28(GANK)
计算分子量 226 aa, 24 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC011960
基因名称 PSMD10
Gene ID (NCBI) 5716
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75832
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMD10, also known as Gankyrin, is a subunit of the 19S regulatory particle of the proteasome and plays a crucial role in protein degradation. It is a small, 25 kDa cytoplasmic-nuclear shuttling protein, highly conserved throughout evolution (~40% identity to yeast Nas6p). PSMD10 is involved in the regulation of cell cycle progression and apoptosis by interacting with key proteins such as p53 and Rb. PSMD10 has been identified as an oncoprotein, promoting tumor cell proliferation and survival in various cancers (PMID: 16581249, PMID: 31576540).