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验证数据展示

  • WB analysis using 86237-1-RR (same clone as 86237-1-PBS)

    WB analysis using 86237-1-RR (same clone as 86237-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86237-1-RR (PSMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 86237-1-RR (same clone as 86237-1-PBS)

    IF Staining of Jurkat using 86237-1-RR (same clone as 86237-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMC1 antibody (86237-1-RR, Clone: 251014B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 86237-1-RR (same clone as 86237-1-PBS)

    IHC staining of mouse cerebellum using 86237-1-RR (same clone as 86237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86237-1-RR (PSMC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMC1 antibody included
  • Affinity and Kinetic Characterization of 86237-1-RR

    Affinity and Kinetic Characterization of 86237-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86237-1-RR against Human PSMC1 were performed. The affinity constant is 0.402 nM.

PSMC1 Recombinant monoclonal antibody, PBS Only

PSMC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86237-1-PBS

产品说明书

CloneNo. 251014B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

26S proteasome AAA-ATPase subunit RPT2, 26S proteasome regulatory subunit 4, P26S4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMC1 Ready-To-Use IHC Kit. PSMC1 primary antibody included.

产品信息

86237-1-PBS targets PSMC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1677

Product name: Recombinant human PSMC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-440 aa of BC016368

Sequence: EFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNRAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, ATPase, 1
别名 26S proteasome AAA-ATPase subunit RPT2, 26S proteasome regulatory subunit 4, P26S4
计算分子量 49 kDa
观测分子量52-60 kDa
GenBank蛋白编号BC016368
基因名称 PSMC1
Gene ID (NCBI) 5700
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62191
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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