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验证数据展示

  • WB analysis using 86491-1-RR (same clone as 86491-1-PBS)

    WB analysis using 86491-1-RR (same clone as 86491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86491-1-RR (PSMB6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86491-1-RR (same clone as 86491-1-PBS)

    IF Staining of HeLa using 86491-1-RR (same clone as 86491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB6 antibody (86491-1-RR, Clone: 251232G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86491-1-RR

    Affinity and Kinetic Characterization of 86491-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86491-1-RR against Human PSMB6 were performed. The affinity constant is below 1 pM.

PSMB6 Recombinant monoclonal antibody, PBS Only

PSMB6 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86491-1-PBS

产品说明书

CloneNo. 251232G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMB6 Ready-To-Use IHC Kit. PSMB6 primary antibody included.

产品信息

86491-1-PBS targets PSMB6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2296

Product name: Recombinant human PSMB6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-239 aa of BC000835

Sequence: MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 6
别名 EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y
计算分子量 239 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC000835
基因名称 PSMB6
Gene ID (NCBI) 5694
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP28072
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

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