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验证数据展示

  • WB analysis of Jurkat using 55143-1-AP (same clone as 55143-1-PBS)

    WB analysis of Jurkat using 55143-1-AP (same clone as 55143-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55143-1-AP (PSMB11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

  • IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

    IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

  • IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

    IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 55143-1-AP (same clone as 55143-1-PBS)

    FC experiment of MCF-7 using 55143-1-AP (same clone as 55143-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PSMB11 (55143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

PSMB11 Polyclonal antibody, PBS Only

PSMB11 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 55143-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55143-1-PBS targets PSMB11 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 11
别名
计算分子量 33 kDa
观测分子量 32-35 kDa
GenBank蛋白编号NM_001099780
基因名称 PSMB11
Gene ID (NCBI) 122706
RRIDAB_10863288
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5LHX3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.

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