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验证数据展示

  • WB analysis of Jurkat using 55143-1-AP

    WB analysis of Jurkat using 55143-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55143-1-AP (PSMB11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thymus using 55143-1-AP

    IHC staining of human thymus using 55143-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thymus using 55143-1-AP

    IHC staining of human thymus using 55143-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of MCF-7 using 55143-1-AP

    FC experiment of MCF-7 using 55143-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PSMB11 (55143-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

PSMB11 Polyclonal antibody

PSMB11 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 55143-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

Proteasome subunit beta-5t, Proteasome subunit beta type-11, EC:3.4.25.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55143-1-AP targets PSMB11 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 11
别名 Proteasome subunit beta-5t, Proteasome subunit beta type-11, EC:3.4.25.1
计算分子量 33 kDa
观测分子量 32-35 kDa
GenBank蛋白编号NM_001099780
基因名称 PSMB11
Gene ID (NCBI) 122706
RRIDAB_10863288
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5LHX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.

实验方案

Product Specific Protocols
WB protocol for PSMB11 antibody 55143-1-APDownload protocol
IHC protocol for PSMB11 antibody 55143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols