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验证数据展示

  • FC experiment of HepG2 using 14022-1-AP

    FC experiment of HepG2 using 14022-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA8 Polyclonal antibody (14022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 14022-1-AP

    WB analysis using 14022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 14022-1-AP

    IHC staining of human testis using 14022-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PSMA8 antibody included
  • IHC staining of human testis using 14022-1-AP

    IHC staining of human testis using 14022-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PSMA8 antibody included
  • IF Staining of HepG2 using 14022-1-AP

    IF Staining of HepG2 using 14022-1-AP

    Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody 14022-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of HeLa using 14022-1-AP

    WB analysis of HeLa using 14022-1-AP

    WB result of PSMA8 antibody (14022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

PSMA8 Polyclonal antibody

PSMA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14022-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Alpha4s, Proteasome alpha 4 subunit, Proteasome subunit alpha-type 7-like, Proteasome subunit alpha-type 8, PSMA7L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, mouse thymus tissue, HeLa cells, rat thymus tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA8 Ready-To-Use IHC Kit. PSMA8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14022-1-AP targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag5138

Product name: Recombinant human PSMA8 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-250 aa of BC042820

Sequence: MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVVINRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTEDAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEKEEAEKKKSKKSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 8
别名 Alpha4s, Proteasome alpha 4 subunit, Proteasome subunit alpha-type 7-like, Proteasome subunit alpha-type 8, PSMA7L
计算分子量 29 kDa
观测分子量28 kDa
GenBank蛋白编号BC042820
基因名称 PSMA8
Gene ID (NCBI) 143471
RRIDAB_10896314
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAA3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMA8 antibody 14022-1-APDownload protocol
IHC protocol for PSMA8 antibody 14022-1-APDownload protocol
IF protocol for PSMA8 antibody 14022-1-APDownload protocol
FC protocol for PSMA8 antibody 14022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis.

Authors - Zhiming Li
View Article
mouseIF

PLoS Genet

The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.

Authors - Laura Gómez-H
View Article
mouseWB

Sci China Life Sci

Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice

Authors - Yiling Zhou
View Article

Zool Res

Both 20S and 19S proteasome components are essential for meiosis in male mice

Authors - Ting-Ting Han
View Article