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验证数据展示

  • WB analysis using 67817-1-Ig (same clone as 67817-1-PBS)

    WB analysis using 67817-1-Ig (same clone as 67817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

    IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA7 antibody included
  • WB analysis of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    WB analysis of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • IF Staining of Caco-2 using 67817-1-Ig (same clone as 67817-1-PBS)

    IF Staining of Caco-2 using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    IF Staining of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

    FC experiment of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug PSMA7 Monoclonal antibody (67817-1-Ig, Clone:1F2C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

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PSMA7 Monoclonal antibody, PBS Only

PSMA7 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67817-1-PBS

产品说明书

CloneNo. 1F2C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMA7 Ready-To-Use IHC Kit. PSMA7 primary antibody included.

产品信息

67817-1-PBS targets PSMA7 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7821

Product name: Recombinant human PSMA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC004427

Sequence: MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 7
别名 C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC004427
基因名称 PSMA7
Gene ID (NCBI) 5688
RRIDAB_2918580
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO14818
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

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