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验证数据展示

  • WB analysis using 31384-1-AP (same clone as 31384-1-PBS)

    WB analysis using 31384-1-AP (same clone as 31384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31384-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 31384-1-AP (same clone as 31384-1-PBS)

    IHC staining of rat liver using 31384-1-AP (same clone as 31384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA5 antibody included
  • IHC staining of mouse brain using 31384-1-AP (same clone as 31384-1-PBS)

    IHC staining of mouse brain using 31384-1-AP (same clone as 31384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA5 antibody included
  • IF Staining of U2OS using 31384-1-AP (same clone as 31384-1-PBS)

    IF Staining of U2OS using 31384-1-AP (same clone as 31384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (31384-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PSMA5 Polyclonal antibody, PBS Only

PSMA5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31384-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Proteasome subunit alpha type-5, PSC5, ZETA

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMA5 Ready-To-Use IHC Kit. PSMA5 primary antibody included.

产品信息

31384-1-PBS targets PSMA5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34928

Product name: Recombinant human PSMA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC103751

Sequence: MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 5
别名 Proteasome subunit alpha type-5, PSC5, ZETA
计算分子量 241 aa, 26 kDa
观测分子量26-30 kDa
GenBank蛋白编号BC103751
基因名称 PSMA5
Gene ID (NCBI) 5686
RRIDAB_3669959
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP28066
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

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