验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse liver tissue, mouse thymus tissue |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy PSMA5 Ready-To-Use IHC Kit. PSMA5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
31384-1-AP targets PSMA5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PSMA5 fusion protein Ag34928 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, alpha type, 5 |
| 别名 | ZETA, PSC5, Proteasome subunit alpha type-5 |
| 计算分子量 | 241 aa, 26 kDa |
| 观测分子量 | 26-30 kDa |
| GenBank蛋白编号 | BC103751 |
| 基因名称 | PSMA5 |
| Gene ID (NCBI) | 5686 |
| RRID | AB_3669959 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P28066 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMA5 antibody 31384-1-AP | Download protocol |
| IF protocol for PSMA5 antibody 31384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |