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验证数据展示

  • WB analysis using 68203-1-Ig (same clone as 68203-1-PBS)

    WB analysis using 68203-1-Ig (same clone as 68203-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 68203-1-Ig (same clone as 68203-1-PBS)

    IHC staining of human pancreas cancer using 68203-1-Ig (same clone as 68203-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA4 antibody included
  • IF Staining of HeLa using 68203-1-Ig (same clone as 68203-1-PBS)

    IF Staining of HeLa using 68203-1-Ig (same clone as 68203-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

PSMA4 Monoclonal antibody, PBS Only

PSMA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68203-1-PBS

产品说明书

CloneNo. 2E6E4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2E6E4, HC9, Proteasome subunit alpha type-4, PSC9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMA4 Ready-To-Use IHC Kit. PSMA4 primary antibody included.

产品信息

68203-1-PBS targets PSMA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag32626

Product name: Recombinant human PSMA4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-237 aa of BC056249

Sequence: MEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 4
别名 2E6E4, HC9, Proteasome subunit alpha type-4, PSC9
计算分子量 237 aa, 27 kDa
观测分子量29 kDa
GenBank蛋白编号BC056249
基因名称 PSMA4
Gene ID (NCBI) 5685
RRIDAB_2935291
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25789
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMA4 (Proteasome Subunit Alpha 4) is a gene that encodes the alpha-4 subunit of the 20S proteasome core particle, which forms part of the outer alpha-ring structure of the barrel-shaped 20S complex. As an integral component of the 26S proteasome, PSMA4 participates in ATP- and ubiquitin-dependent protein degradation, a process essential for the regulation of cell cycle progression, signal transduction, and protein quality control. In addition, the 20S core particle can associate with regulatory complexes such as PA28 or PA200 to mediate ubiquitin-independent protein degradation, which plays important roles in pathways including antigen peptide generation and spermatogenesis. Dysregulation of PSMA4 expression or function has been implicated in various pathological conditions, including cancer and neurodegenerative diseases, highlighting its critical role in maintaining cellular proteostasis.

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