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验证数据展示

  • WB analysis using 67938-1-Ig

    WB analysis using 67938-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67938-1-Ig (PSMA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67938-1-Ig

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA3 antibody included
  • IHC staining of human liver cancer using 67938-1-Ig

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA3 antibody included

PSMA3 Monoclonal antibody

PSMA3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67938-1-Ig

产品说明书

CloneNo. 1A8C9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA3 Ready-To-Use IHC Kit. PSMA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67938-1-Ig targets PSMA3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag30552

Product name: Recombinant human PSMA3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-255 aa of BC029402

Sequence: MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADMAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 3
别名 HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3
计算分子量 255 aa, 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC029402
基因名称 PSMA3
Gene ID (NCBI) 5684
RRIDAB_2918690
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMA3 antibody 67938-1-IgDownload protocol
IHC protocol for PSMA3 antibody 67938-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols