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PSMA2 Polyclonal antibody

PSMA2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14377-1-AP

Print datasheet

Synonyms

20S proteasome, HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2

验证数据展示

  • IHC staining of human skeletal muscle using 14377-1-AP

    IHC staining of human skeletal muscle using 14377-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • IHC staining of human skeletal muscle using 14377-1-AP

    IHC staining of human skeletal muscle using 14377-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • WB analysis of NIH/3T3 using 14377-1-AP

    WB analysis of NIH/3T3 using 14377-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14377-1-AP

    IF Staining of HepG2 using 14377-1-AP

    Immunofluorescent analysis of HepG2 cells using 14377-1-AP (PSMA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, PC-3 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA2 Ready-To-Use IHC Kit. PSMA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14377-1-AP targets PSMA2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PSMA2 fusion protein Ag5753 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 2
Synonyms 20S proteasome, HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC047697
Gene Symbol PSMA2
Gene ID (NCBI) 5683
RRIDAB_2878050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

实验方案

Product Specific Protocols
WB protocol for PSMA2 antibody 14377-1-APDownload protocol
IHC protocol for PSMA2 antibody 14377-1-APDownload protocol
IF protocol for PSMA2 antibody 14377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Biomater

Pushpin-like nanozyme for plasmon-enhanced tumor targeted therapy

Authors - Baofu Ma
View Article
humanIHC

J Ovarian Res

Development and clinical validation of a seven-gene signature based on tumor stem cell-related genes to predict ovarian cancer prognosis

Authors - Guangwei Wang
View Article