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PSEN1 Polyclonal antibody

PSEN1 Polyclonal Antibody for IF/ICC,IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC,IF-P, IHC, ELISA

Conjugate

Unconjugated

Cat No : 20434-1-AP

Print datasheet

Synonyms

AD3, FAD, presenilin 1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182

验证数据展示

  • IF Staining of mouse brain using 20434-1-AP

    IF Staining of mouse brain using 20434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSEN1 antibody (20434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 20434-1-AP

    IF Staining of mouse brain using 20434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSEN1 antibody (20434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human gliomas using 20434-1-AP

    IHC staining of human gliomas using 20434-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20434-1-AP (PSEN1 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human gliomas using 20434-1-AP

    IHC staining of human gliomas using 20434-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20434-1-AP (PSEN1 Antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HEK-293 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

20434-1-AP targets PSEN1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC,IF-P, IHC, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen PSEN1 fusion protein Ag14135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name presenilin 1
Synonyms AD3, FAD, presenilin 1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182
Calculated Molecular Weight 467 aa, 53 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC011729
Gene Symbol PSEN1
Gene ID (NCBI) 5663
RRIDAB_2878685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a ~30-kDa N-terminal fragment (NTF) and a ~20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody can recognize all the isoforms except isoform 4 (21 kDa).

实验方案

Product Specific Protocols
IHC protocol for PSEN1 antibody 20434-1-APDownload protocol
IF protocol for PSEN1 antibody 20434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Front Aging Neurosci

LongShengZhi Capsule Attenuates Alzheimer-Like Pathology in APP/PS1 Double Transgenic Mice by Reducing Neuronal Oxidative Stress and Inflammation.

Authors - Zequn Yin
View Article