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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PSAT1 Polyclonal antibody

PSAT1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10501

Print datasheet

Synonyms

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

验证数据展示

  • FC experiment of HepG2 using CL488-10501

    FC experiment of HepG2 using CL488-10501

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PSAT1 (CL488-10501) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10501-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10501 targets PSAT1 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, rat
Immunogen PSAT1 fusion protein Ag0776 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoserine aminotransferase 1
Synonyms EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC004863
Gene Symbol PSAT1
Gene ID (NCBI) 29968
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y617
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PSAT1 antibody CL488-10501Download protocol
Standard Protocols
Click here to view our Standard Protocols