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PSAP Polyclonal antibody

PSAP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18398-1-AP

Print datasheet

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

验证数据展示

  • WB analysis of HeLa using 18398-1-AP

    WB analysis of HeLa using 18398-1-AP

    WB result of PSAP antibody (18398-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells.

  • WB analysis of HeLa using 18398-1-AP

    WB analysis of HeLa using 18398-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18398-1-AP (PSAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18398-1-AP

    IHC staining of human liver cancer using 18398-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18398-1-AP (PSAP antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of human liver cancer using 18398-1-AP

    IHC staining of human liver cancer using 18398-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18398-1-AP (PSAP antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PSAP antibody included
  • IF Staining of PC-3 using 18398-1-AP

    IF Staining of PC-3 using 18398-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 18398-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSAP Ready-To-Use IHC Kit. PSAP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18398-1-AP targets PSAP in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen PSAP fusion protein Ag13265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prosaposin
Synonyms A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_10598315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin C.

实验方案

Product Specific Protocols
WB protocol for PSAP antibody 18398-1-APDownload protocol
IHC protocol for PSAP antibody 18398-1-APDownload protocol
IF protocol for PSAP antibody 18398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Hum Mol Genet

Lysosomal ceramides regulate Cathepsin B-mediated processing of saposin C and glucocerebrosidase activity.

Authors - Myung Jong Kim
View Article