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PRY Polyclonal antibody

PRY Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24688-1-AP

Print datasheet

Synonyms

PRY, PRY2, PRYP3, PRYP4, PTPN13 like protein, Y linked, PTPN13 like, Y linked 2, PTPN13LY, PTPN13LY2

验证数据展示

  • IF Staining of HepG2 using 24688-1-AP

    IF Staining of HepG2 using 24688-1-AP

    Immunofluorescent analysis of HepG2 cells using 24688-1-AP (PRY antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • WB analysis of PC-3 using 24688-1-AP

    WB analysis of PC-3 using 24688-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24688-1-AP (PRY antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24688-1-AP is PRY Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen PRY fusion protein Ag20232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PTPN13-like, Y-linked 2
Synonyms PRY, PRY2, PRYP3, PRYP4, PTPN13 like protein, Y linked, PTPN13 like, Y linked 2, PTPN13LY, PTPN13LY2
Calculated Molecular Weight 147 aa, 17 kDa
GenBank Accession NumberBC153128
Gene Symbol PRY2
Gene ID (NCBI) 442862
RRIDAB_2879672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14603
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRY is Testis-specific PTP-BL-related Y protein which has two isoforms with MW 16 and 6-8 kDa.

实验方案

Product Specific Protocols
WB protocol for PRY antibody 24688-1-APDownload protocol
IHC protocol for PRY antibody 24688-1-APDownload protocol
IF protocol for PRY antibody 24688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols