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验证数据展示

  • WB analysis of human testis using 24688-1-AP

    WB analysis of human testis using 24688-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24688-1-AP (PRY antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 24688-1-AP

    IHC staining of human prostate cancer using 24688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24688-1-AP (PRY antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human prostate cancer using 24688-1-AP

    IHC staining of human prostate cancer using 24688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24688-1-AP (PRY antibody at dilution of 1:200 (under 40x lens).

  • IF Staining of PC-3 using 24688-1-AP

    IF Staining of PC-3 using 24688-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PRY antibody (24688-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

PRY Polyclonal antibody

PRY Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24688-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PRY2, PRY1, PRYP3, PRYP4, PTPN13 like protein, Y linked

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24688-1-AP targets PRY in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PRY fusion protein Ag20232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PTPN13-like, Y-linked 2
别名 PRY2, PRY1, PRYP3, PRYP4, PTPN13 like protein, Y linked
计算分子量 147 aa, 17 kDa
观测分子量7 kDa
GenBank蛋白编号BC153128
基因名称 PRY
Gene ID (NCBI) 442862
RRIDAB_2879672
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14603
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRY is Testis-specific PTP-BL-related Y protein which has two isoforms with MW 16 and 6-8 kDa.

实验方案

Product Specific Protocols
WB protocol for PRY antibody 24688-1-APDownload protocol
IHC protocol for PRY antibody 24688-1-APDownload protocol
IF protocol for PRY antibody 24688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols