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验证数据展示

  • IF Staining of HeLa using CL488-67599

    IF Staining of HeLa using CL488-67599

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PRX5 antibody (CL488-67599, Clone: 1B8C5 ) at dilution of 1:600.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PRX5 Monoclonal antibody

PRX5 Monoclonal Antibody for IF/ICC
Cat No. CL488-67599

产品说明书

CloneNo. 1B8C5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC

别名

Peroxiredoxin 5, PRDX5, B166, AOPP, AOEB166

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRDX5 Ready-To-Use IHC Kit. PRDX5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67599-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67599 targets PRX5 in IF/ICC applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, rat
免疫原 PRX5 fusion protein Ag12262 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 5
别名 Peroxiredoxin 5, PRDX5, B166, AOPP, AOEB166
计算分子量 214 aa, 22 kDa
观测分子量 17 kDa
GenBank蛋白编号BC110983
基因名称 PRX5
Gene ID (NCBI) 25824
RRIDAB_3672954
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30044
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRX5 antibody CL488-67599Download protocol
Standard Protocols
Click here to view our Standard Protocols