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CoraLite® Plus 488-conjugated PRDX3 Monoclonal antibody

PRDX3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F7F7

Cat No : CL488-66810

Print datasheet

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

验证数据展示

  • FC experiment of MCF-7 using CL488-66810

    FC experiment of MCF-7 using CL488-66810

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRX3 (CL488-66810, Clone:1F7F7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66810-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66810 targets PRDX3 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen PRDX3 fusion protein Ag1062 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 3
Synonyms Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30048
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PRDX3 antibody CL488-66810Download protocol
Standard Protocols
Click here to view our Standard Protocols