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验证数据展示

  • IHC staining of human lung cancer using 17392-1-AP

    IHC staining of human lung cancer using 17392-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17392-1-AP (PRPF40A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRPF40A antibody included
  • IHC staining of human lung cancer using 17392-1-AP

    IHC staining of human lung cancer using 17392-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17392-1-AP (PRPF40A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRPF40A antibody included
  • WB analysis of HEK-293 using 17392-1-AP

    WB analysis of HEK-293 using 17392-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 17392-1-AP

    IP experiment of HEK-293 using 17392-1-AP

    IP result of anti-PRPF40A (IP:17392-1-AP, 4ug; Detection:17392-1-AP 1:500) with HEK-293 cells lysate 3000ug.

PRPF40A Polyclonal antibody

PRPF40A Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

Fas ligand associated factor 1, Fas ligand-associated factor 1, FBP 11, FBP11, FLAF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRPF40A Ready-To-Use IHC Kit. PRPF40A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17392-1-AP targets PRPF40A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11290

Product name: Recombinant human PRPF40A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 743-957 aa of BC027178

Sequence: MKRKESAFKSMLKQAAPPIELDAVWEDIRERFVKEPAFEDITLESERKRIFKDFMHVLEHECQHHHSKNKKHSKKSKKHHRKRSRSRSGSDSDDDDSHSKKKRQRSESRSASEHSSSAESERSYKKSKKHKKKSKKRRHKSDSPESDAEREKDKKEKDRESEKDRTRQRSESKHKSPKKKTGKDSGNWDTSGSELSEGELEKRRRTLLEQLDDDQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)
别名 Fas ligand associated factor 1, Fas ligand-associated factor 1, FBP 11, FBP11, FLAF1
计算分子量 957 aa, 109 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC027178
基因名称 PRPF40A
Gene ID (NCBI) 55660
RRIDAB_2171031
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75400
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for PRPF40A antibody 17392-1-APDownload protocol
IP protocol for PRPF40A antibody 17392-1-APDownload protocol
WB protocol for PRPF40A antibody 17392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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