PRPF4 Polyclonal antibody

PRPF4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10728-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

HPRP4, HPRP4P, PRP4, PRP4 homolog, Prp4p, PRPF4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, Raji cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

10728-1-AP targets PRPF4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag1193

Product name: Recombinant human PRPF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 213-522 aa of BC001588

Sequence: ELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP4 pre-mRNA processing factor 4 homolog (yeast)
别名 HPRP4, HPRP4P, PRP4, PRP4 homolog, Prp4p, PRPF4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC001588
基因名称 PRPF4
Gene ID (NCBI) 9128
RRIDAB_2171027
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43172
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPF4, also named as U4/U6 small nuclear ribonucleoprotein Prp4, is a 522 amino acid protein, which contains seven WD repeats. PRPF4 localizes in the Nucleus speckle. PRPF4 participates in pre-mRNA splicing and is a part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.PRPF4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes

实验方案

Product Specific Protocols
WB protocol for PRPF4 antibody 10728-1-APDownload protocol
IHC protocol for PRPF4 antibody 10728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
humanWB

Hum Mol Genet

PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.

Authors - Tanackovic Goranka G
mouseWB

J Biol Chem

Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture.

Authors - Li Ming M
humanWB

Cell Rep Med

Clinical-proteomic classification and precision treatment strategy of chordoma

Authors - Huabin Yin
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