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验证数据展示

  • WB analysis using 86315-2-RR (same clone as 86315-2-PBS)

    WB analysis using 86315-2-RR (same clone as 86315-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86315-2-RR (PRPF31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86315-2-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 86315-2-RR (same clone as 86315-2-PBS)

    IF Staining of Jurkat using 86315-2-RR (same clone as 86315-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86315-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86315-2-RR (same clone as 86315-2-PBS)

    IF Staining of HeLa using 86315-2-RR (same clone as 86315-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86315-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86315-2-RR

    Affinity and Kinetic Characterization of 86315-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86315-2-RR against Human PRPF31 were performed. The affinity constant is below 1 pM.

PRPF31 Recombinant monoclonal antibody, PBS Only

PRPF31 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86315-2-PBS

产品说明书

CloneNo. 251005H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

hPrp31, NY BR 99, Protein 61K, PRP31, RP11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86315-2-PBS targets PRPF31 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26716

Product name: Recombinant human PRPF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-212 aa of NM_015629

Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
别名 hPrp31, NY BR 99, Protein 61K, PRP31, RP11
计算分子量 55 kDa
观测分子量55-60 kDa
GenBank蛋白编号NM_015629
基因名称 PRPF31
Gene ID (NCBI) 26121
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WWY3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

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