×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 27750-1-AP (same clone as 27750-1-PBS)

    WB analysis using 27750-1-AP (same clone as 27750-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27750-1-AP (PRPF31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRPF31 antibody included
  • IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRPF31 antibody included
  • IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

PRPF31 Polyclonal antibody, PBS Only

PRPF31 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 27750-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy® PRPF31 Ready-To-Use IHC Kit. PRPF31 primary antibody included.

产品信息

27750-1-PBS targets PRPF31 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26716

Product name: Recombinant human PRPF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-212 aa of NM_015629

Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
别名
计算分子量 55 kDa
观测分子量55 kDa
GenBank蛋白编号NM_015629
基因名称 PRPF31
Gene ID (NCBI) 26121
RRIDAB_2918130
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWY3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

||
New chat

Able™

正在加载，请稍候...