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PRPF31 Polyclonal antibody

PRPF31 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27750-1-AP

Print datasheet

Synonyms

hPrp31, NY BR 99, Pre mRNA processing factor 31, Protein 61K, PRP31, PRPF31, RP11, U4/U6 snRNP 61 kDa protein

验证数据展示

  • WB analysis using 27750-1-AP

    WB analysis using 27750-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27750-1-AP (PRPF31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 27750-1-AP

    IHC staining of mouse eye using 27750-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 27750-1-AP

    IHC staining of mouse eye using 27750-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 27750-1-AP

    IF Staining of mouse eye using 27750-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 27750-1-AP

    IF Staining of mouse eye using 27750-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, Caco-2 cells, Hela cells, Jurkat cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27750-1-AP targets PRPF31 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen PRPF31 fusion protein Ag26716 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
Synonyms hPrp31, NY BR 99, Pre mRNA processing factor 31, Protein 61K, PRP31, PRPF31, RP11, U4/U6 snRNP 61 kDa protein
Calculated Molecular Weight 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberNM_015629
Gene Symbol PRPF31
Gene ID (NCBI) 26121
RRIDAB_2918130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

实验方案

Product Specific Protocols
WB protocol for PRPF31 antibody 27750-1-APDownload protocol
IHC protocol for PRPF31 antibody 27750-1-APDownload protocol
IF protocol for PRPF31 antibody 27750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols