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验证数据展示

  • WB analysis using 83831-5-RR

    WB analysis using 83831-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83831-5-RR (PROSC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83831-5-RR

    IHC staining of mouse liver using 83831-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83831-5-RR (PROSC antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83831-5-RR

    Affinity and Kinetic Characterization of 83831-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83831-5-RR against Human PROSC were performed. The affinity constant is 2.00 nM.

PROSC Recombinant antibody

PROSC Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83831-5-RR

产品说明书

CloneNo. 240998B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PLPBP, 240998B6, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, Jurkat cells, L02 cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:325-1:1300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83831-5-RR targets PROSC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PROSC fusion protein Ag18369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proline synthetase co-transcribed homolog (bacterial)
别名 PLPBP, 240998B6, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein
计算分子量 275 aa, 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012334
基因名称 PROSC
Gene ID (NCBI) 11212
RRIDAB_3671415
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO94903
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).

实验方案

Product Specific Protocols
WB protocol for PROSC antibody 83831-5-RRDownload protocol
IHC protocol for PROSC antibody 83831-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols