验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, Jurkat cells, L02 cells, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83831-5-RR targets PROSC in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PROSC fusion protein Ag18369 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | proline synthetase co-transcribed homolog (bacterial) |
| 别名 | PLPBP, Pyridoxal phosphate-binding protein, Pyridoxal phosphate homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, PLP homeostasis protein |
| 计算分子量 | 275 aa, 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC012334 |
| 基因名称 | PROSC |
| Gene ID (NCBI) | 11212 |
| RRID | AB_3671415 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O94903 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PROSC antibody 83831-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |