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验证数据展示

  • WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)

    WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • WB analysis using 16910-1-AP (same clone as 16910-1-PBS)

    WB analysis using 16910-1-AP (same clone as 16910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

    IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

    IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

    FC experiment of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

    IF Staining of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (16910-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

PROS1 Polyclonal antibody, PBS Only

PROS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 16910-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

PROS, Vitamin K-dependent protein S

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16910-1-PBS targets PROS1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10539

Product name: Recombinant human PROS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 328-676 aa of BC015801

Sequence: DFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein S (alpha)
别名 PROS, Vitamin K-dependent protein S
计算分子量 75 kDa
观测分子量 70 kDa
GenBank蛋白编号BC015801
基因名称 PROS1
Gene ID (NCBI) 5627
RRIDAB_2300207
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07225
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

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