验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68621-1-Ig targets PRODH in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat, rabbit, pig |
| 免疫原 | PRODH fusion protein Ag18429 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | proline dehydrogenase (oxidase) 1 |
| 别名 | FLJ33744, HSPOX2, p53 induced gene 6 protein, PIG6, POX, POX2, PRODH, PRODH1, PRODH2, Proline oxidase, Proline oxidase 2, SCZD4, TP53I6 |
| 计算分子量 | 600 aa, 68 kDa |
| 观测分子量 | 56 kDa, 66 kDa |
| GenBank蛋白编号 | BC118597 |
| 基因名称 | PRODH |
| Gene ID (NCBI) | 5625 |
| RRID | AB_3085313 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43272 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRODH(Proline dehydrogenase 1, mitochondrial) is also named as PIG6, HSPOX2, PRODH1, PRODH2, POX, SCZD4, TP53I6 and belongs to the proline oxidase family. It is an oxidoreductase involved in the transfer of redox potential across the mitochondrial membrane and catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C). High PRODH activity is sufficient to induce mitochondria-mediated apoptosis in the presence of proline. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) and defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). This protein has 3 isoforms produced by alternative splicing with the molecular mass of 68 kDa, 59 kDa and 56 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRODH antibody 68621-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |