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验证数据展示

  • WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

    WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

    Non-treated mouse testis and glucosidase treated mouse testis tissue were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

  • WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

    WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

    IF Staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

    IHC staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

PRND Polyclonal antibody, PBS Only

PRND Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 26947-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26947-1-PBS targets PRND in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25523

Product name: Recombinant human PRND protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-176 aa of BC043644

Sequence: GIKHRIKWNRKALPSTAQITEAQVAENRPGAFIKQGRKLDIDFGAEGNRYYEANYWQFPDGIHYNGCSEANVTKEAFVTGCINATQAANQGEFQKPDNKLHQQVLWRLVQELCSLKHCEFWLERGAGLRVTMHQPVLLCLLALIWLMVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prion protein 2 (dublet)
别名
计算分子量 176 aa, 20 kDa
观测分子量17-20, 30 kDa
GenBank蛋白编号BC043644
基因名称 PRND
Gene ID (NCBI) 23627
RRIDAB_2880695
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKY0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The prion gene family is mainly composed of three genes; PRNP, encoding the prion protein (PrP), PRND, encoding Doppel (Dpl), both located on the same genomic locus on mouse chromosome 2 and SPRN, encoding Shadoo (Sho), located on mouse chromosome 7. PRND (prion protein 2 also called Doppel) is a membranean N-glycosylated, glycosylphosphatidylinositol-anchored protein that is found predominantly in testis. PRND gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prionprotein, to which it is biochemically and structurally similar. 26947-1-AP antibody detects a 30 kDa glycosylated protein and a 17-20 kDa protein treated by glucosidase.

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