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PRND Polyclonal antibody

PRND Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26947-1-AP

Print datasheet

Synonyms

DOPPEL, DPL, Prion like protein doppel, Prion protein 2, prion protein 2 (dublet), PRND, PrPLP

验证数据展示

  • WB analysis using 26947-1-AP

    WB analysis using 26947-1-AP

    Non-treated mouse testis and glucosidase treated mouse testis tissue were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 26947-1-AP

    WB analysis using 26947-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 26947-1-AP

    IF Staining of mouse testis using 26947-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 26947-1-AP

    IHC staining of mouse testis using 26947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inGlucosidase treated mouse testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 26947-1-AP is PRND Fusion Protein expressed in E. coli.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, Rat
Cited Reactivitypig
Immunogen PRND fusion protein Ag25523 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prion protein 2 (dublet)
Synonyms DOPPEL, DPL, Prion like protein doppel, Prion protein 2, prion protein 2 (dublet), PRND, PrPLP
Calculated Molecular Weight 176 aa, 20 kDa
Observed Molecular Weight17-20, 30 kDa
GenBank Accession NumberBC043644
Gene Symbol PRND
Gene ID (NCBI) 23627
RRIDAB_2880695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The prion gene family is mainly composed of three genes; PRNP, encoding the prion protein (PrP), PRND, encoding Doppel (Dpl), both located on the same genomic locus on mouse chromosome 2 and SPRN, encoding Shadoo (Sho), located on mouse chromosome 7. PRND (prion protein 2 also called Doppel) is a membranean N-glycosylated, glycosylphosphatidylinositol-anchored protein that is found predominantly in testis. PRND gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prionprotein, to which it is biochemically and structurally similar. 26947-1-AP antibody detects a 30 kDa glycosylated protein and a 17-20 kDa protein treated by glucosidase.

实验方案

Product Specific Protocols
WB protocol for PRND antibody 26947-1-APDownload protocol
IHC protocol for PRND antibody 26947-1-APDownload protocol
IF protocol for PRND antibody 26947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

J Genet Genomics

Single-cell RNA-seq analysis of testicular somatic cell development in pigs.

Authors - Lingkai Zhang
View Article