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PRMT6 Polyclonal antibody
PRMT6 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, MCF-7 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human prostate cancer tissue, human kidney tissue, human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15395-1-AP targets PRMT6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | PRMT6 fusion protein Ag7592 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein arginine methyltransferase 6 |
Synonyms | HRMT1L6, PRMT6 |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC002729 |
Gene Symbol | PRMT6 |
Gene ID (NCBI) | 55170 |
RRID | AB_2237723 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96LA8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PRMT6 antibody 15395-1-AP | Download protocol |
IHC protocol for PRMT6 antibody 15395-1-AP | Download protocol |
IF protocol for PRMT6 antibody 15395-1-AP | Download protocol |
IP protocol for PRMT6 antibody 15395-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO Rep Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis.
| ||
PLoS Pathog SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy. | ||
Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. | ||
Aging (Albany NY) Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling. | ||
Front Cell Dev Biol F-Box Protein FBXW17-Mediated Proteasomal Degradation of Protein Methyltransferase PRMT6 Exaggerates CSE-Induced Lung Epithelial Inflammation and Apoptosis. | ||