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验证数据展示

  • WB analysis using 83969-7-RR

    WB analysis using 83969-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83969-7-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83969-7-RR

    IF Staining of HeLa using 83969-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (83969-7-RR, Clone: 241092E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83969-7-RR

    FC experiment of A549 using 83969-7-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-7-RR, Clone:241092E6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

PRMT3 Recombinant monoclonal antibody

PRMT3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83969-7-RR

产品说明书

CloneNo. 241092E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, SW480 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83969-7-RR targets PRMT3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13466

Product name: Recombinant human PRMT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-531 aa of BC037544

Sequence: RAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein arginine methyltransferase 3
别名 Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319
计算分子量 531 aa, 60 kDa
观测分子量60-68 kDa
GenBank蛋白编号BC037544
基因名称 PRMT3
Gene ID (NCBI) 10196
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.

实验方案

Product Specific Protocols
FC protocol for PRMT3 antibody 83969-7-RRDownload protocol
IF protocol for PRMT3 antibody 83969-7-RRDownload protocol
WB protocol for PRMT3 antibody 83969-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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