验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse liver tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17628-1-AP targets PRMT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, chicken |
免疫原 | PRMT3 fusion protein Ag11877 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | protein arginine methyltransferase 3 |
别名 | Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319 |
计算分子量 | 531 aa, 60 kDa |
观测分子量 | 68 kDa |
GenBank蛋白编号 | BC037544 |
基因名称 | PRMT3 |
Gene ID (NCBI) | 10196 |
RRID | AB_2171654 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O60678 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.
实验方案
Product Specific Protocols | |
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WB protocol for PRMT3 antibody 17628-1-AP | Download protocol |
IHC protocol for PRMT3 antibody 17628-1-AP | Download protocol |
IF protocol for PRMT3 antibody 17628-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Med PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease | ||
Toxicol Appl Pharmacol Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5 | ||
Basic Clin Pharmacol Toxicol Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5. | ||
Biomed Res Shotgun proteomic investigation of methyltransferase and methylation profiles in lipopolysaccharide stimulated RAW264.7 murine macrophages. | ||
Nat Commun Targeting PRMT3 impairs methylation and oligomerization of HSP60 to boost anti-tumor immunity by activating cGAS/STING signaling |